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Exosomal whole transcriptome sequencing is a cutting-edge technique that provides comprehensive analysis of the entire transcriptome contained within exosomes. These small vesicles, secreted by various cell types, encapsulate a wide range of RNA molecules, including mRNA, microRNA, and other non-coding RNAs. By employing exosomal whole transcriptome sequencing, researchers can gain in-depth insights into the molecular mechanisms and communication involved in various physiological and pathological processes, such as cancer progression, metastasis, and immune responses.